Services · Cell Isolation & Processing
Cell isolation & processing.
How donor-source tissue is converted into a research-grade cell preparation matters as much as the source tissue itself. The protocols below are applied across the upstream supply chain.
Isolation protocols
- Gentle mechanical or enzymatic dissociation appropriate to tissue type
- Density-gradient separation for mononuclear and progenitor populations
- Lineage-marker enrichment (CD34, CD45, etc.) where the SKU calls for it
- Native cell preparation without intermediate culture amplification
- Culture expansion only where the SKU is explicitly labeled cultured
Cryopreservation
- Defined cryoprotectant formulation (typically 5–10% DMSO with protein)
- Controlled-rate freezing
- Vapor-phase liquid nitrogen storage at –150 °C or below
- Recommended thaw and recovery procedure documented on the COA
EV isolation (for ExCyte)
- Tangential flow filtration (TFF) plus size-exclusion chromatography (SEC) by default
- Alternative isolation methods available where the research question requires
- Endotoxin testing on every batch
- Particle-to-protein ratio reported per lot